Izolace a stanovení laktoferinu z lidských slin

Abstrakt

Lactoferrin, a globular glycoprotein, is an important component of saliva. It shows an antibacterial, anticancerogenic and anti-inflammatory activity. The aim of this study was to develop a method of isolation of lactoferrin from human saliva using ion exchange chromatography in a monolithic column and spectrometric detection with Pyrogallol Red by the Bradford and biuret methods. The calibration curve for lactoferrin was linear in the range 0.06–62.5 μg ml^–1, limit of detection 0.01 μg ml^–1. The lactoferin concentration in saliva of healthy subjects was 42±4 μg mg^–1. Patient with celiac disease showed 2.5×times higher concentration of lactoferrin compared with healthy subjects.